SMAD1 (Phospho) [pS463/pS465] Human InstantOne ELISA Kit/人SMAD1 (Phospho) [pS463/pS465] InstantOne ELISA试剂盒
货号：85-86182-11
规格：96 tests
价格：6634
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | SMAD1 |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 4086 |
| Gene symbol | SMAD1 |
Gene aliases | BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 |
UniProt ID | (Human) Q15797 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human SMAD1 (Ser463/465) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA专为精确测量细胞裂解物中磷酸化的人SMAD1（Ser463 / 465）而设计。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。SMAD1 is a member of a family of transcription factors involved in mediating signaling by growth and differentiation factors such as transforming growth factor beta (TGF-b) and bone morphogenic protein (BMP).SMAD1是转录因子家族的成员，该家族参与通过生长和分化因子（例如转化生长因子β（TGF-b）和骨形态发生蛋白（BMP））介导信号传导。Target information 靶标信息The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.该基因编码的蛋白质属于SMAD，是与果蝇基因“抗十足瘫痪母亲”（Mad）和秀丽隐杆线虫基因Sma的基因产物相似的蛋白质家族。 SMAD蛋白是介导多种信号通路的信号转导子和转录调节子。该蛋白介导了骨形态发生蛋白（BMP）的信号，该信号参与了一系列生物活动，包括细胞生长，凋亡，形态发生，发育和免疫反应。响应BMP配体，该蛋白可以被BMP受体激酶磷酸化并激活。该蛋白的磷酸化形式与SMAD4形成复合物，这对于其在转录调控中的功能很重要。该蛋白是SMAD特异性E3泛素连接酶（如SMURF1和SMURF2）的靶标，并经过泛素化和蛋白酶体介导的降解。已经观察到编码相同蛋白质的选择性剪接的转录变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| SMAD1 (Phospho) [pS463/pS465] Human InstantOne™ ELISA KitRepresentative data for SMAD1 (Phospho) [pS463/pS465] Multispecies InstantOne™ ELISA. C2C12 cells were either treated with 50 mM dosomorphin for 60 min (-) or stimulted with 10 ng/mL BMP4 for 30 min (+). Lysates were prepared as described in the InstantOne™ User Manual. Phosphorylated SMAD1 (Ser463/465) was analyzed in cell lysates by immunoblotting (left) and InstantOne™ ELISA (right) using 10 µg/analysis. The intensity of the band as detected by immunoblot correlates with InstantOne™ ELISA absorption values.SMAD1（磷酸）[pS463 / pS465]多物种InstantOne™ELISA的代表性数据。 C2C12细胞用50 mM吗啡吗啡处理60分钟（-）或用10 ng / mL BMP4刺激30分钟（+）。 如InstantOne™用户手册中所述制备裂解液。 通过免疫印迹（左）和InstantOne™ELISA（右）使用10 µg /分析法分析细胞裂解物中的磷酸化SMAD1（Ser463 / 465）。 通过免疫印迹检测的条带强度与InstantOne™ELISA吸收值相关。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20668.htm
QQ:1749072012