IkB alpha (Total/Phospho) Human InstantOne ELISA Kit/人IkB alpha (Total/Phospho) InstantOne ELISA试剂盒
货号：85-86063-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | IkB alpha |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 4792 |
| Gene symbol | NFKBIA |
Gene aliases | IKBA, MAD-3, NFKBI |
UniProt ID | (Human) P25963 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human IkBa in cell lysates. Detection of mouse and rat IkBa is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解液中总磷酸化的人IkBa的。 期望检测小鼠和大鼠的IkBa。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.IkB-alpha是一种40 kDa的蛋白质，具有抑制NF- kappaB活性的功能。抑制是通过胞浆中IκB蛋白与NFκB二聚体之间的蛋白相互作用而发生的。 IκB-α与NFκB的相互作用掩盖了NFκB的核定位序列，防止了NFκB易位至核。多种刺激可通过I kappaBα的降解释放NF- kappaB来激活基因表达。这些刺激包括促炎细胞因子TNF-α和IL-1β，趋化因子，PMA，生长因子，LPS，紫外线照射，病毒感染以及各种化学和物理应激。在人类中，该基因位于14号染色体的q臂上。NFkB的激活要求IkB在特定的丝氨酸残基上被磷酸化，从而导致IkB的靶向降解。 IkB激酶alpha（IKK alpha），以前称为CHUK，与IkB-alpha相互作用，并且在触发其降解丝氨酸32和36的位点上特异性磷酸化IkB-alpha。IKKalpha对于响应促炎性细胞因子的NFkB活化似乎至关重要。 NFkB诱导激酶（NIK）刺激了IKKB对IkB的磷酸化，该激酶本身是NFkB激活以响应TNF和IL-1的中央调节剂。功能性IKK复合物包含三个亚基，IKK alpha，IKK beta和IKK gamma，每个似乎都对IkB磷酸化做出了重要贡献。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| IkB alpha (Total/Phospho) Human InstantOne™ ELISA KitRepresentative data for IkB alpha (Total/Phospho) Human InstantOne™ ELISA. HeLa cells were unstimulated (-) or stimulated with 10 ng/mL TNF alpha for 5 min (+). Cells were analyzed for total (left) or phosphorylated (right) IkBa by InstantOne™ ELISA.IkB alpha（总/磷酸）人InstantOne™ELISA的代表性数据。 未刺激（-）或用10 ng / mL TNFα刺激HeLa细胞5分钟（+）。 通过InstantOne™ELISA分析细胞的总（左）或磷酸化（右）IkBa。 |
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