| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated AKT 1/2/3 (Ser473) (human, mouse, hamster, rat), phosphorylated GSK3beta (Ser9) (human, mouse, rat) and phosphorylated p70S6K (Thr389) (human, mouse) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA专为精确测量磷酸化的AKT 1/2/3（Ser473）（人类，小鼠，仓鼠，大鼠），磷酸化的GSK3beta（Ser9）（人类，小鼠，大鼠）和磷酸化的p70S6K（Thr389）（ 人，小鼠）细胞裂解物中。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. AKT phosphorylates over 50 known substrates, including GSK3b, p70S6K, AS160, PRAS40, TSC 1, TCS 2, Raf-1, Bad, the FOXO family of transcription factors, and PFK2. Due to its established critical role in numerous biological processes, AKT is a frequent target for oncology-related drug development.AKT以多种同工型形式存在，是被磷酸肌醇3激酶（PI3K）激活的主要激酶之一。 PI3K的激活导致生成磷脂酰肌醇（3,4,5）-三磷酸（PIP3）。 这些脂质第二信使结合至AKT的pleckstrin同源结构域，以促进其易位至质膜，以分别通过PDK1和mTOR TORC2复合物在Thr308和Ser473处的磷酸化激活。 这两个位点的磷酸化是AKT Ser / Thr激酶活性完全激活所必需的。 AKT使50多种已知底物磷酸化，包括GSK3b，p70S6K，AS160，PRAS40，TSC 1，TCS 2，Raf-1，Bad，FOXO转录因子家族和PFK2。 由于AKT在众多生物学过程中已确立了关键作用，因此它一直是肿瘤相关药物开发的常见目标。Glycogen Synthase Kinase 3 (GSK3) is a Ser/Thr kinase that exists as two isoforms, GSK3a and GSK3ß. Unlike other kinases, phosphorylation at Ser9 and Ser21 for GSK3alpha and GSK3beta, respectively, leads to inactivation of this enzyme. GSK3 has been implicated in several diseases, including cancer and diabetes, through its phosphorylation of glycogen synthase. GSK3 has also been associated with neurodegenerative diseases such as Alzheimer's disease due to its phosphorylation of the neuronal Tau protein.糖原合酶激酶3（GSK3）是一种Ser / Thr激酶，以两种同种型存在，即GSK3a和GSK3ß。 与其他激酶不同，GSK3alpha和GSK3beta分别在Ser9和Ser21处磷酸化导致该酶失活。 GSK3通过其糖原合酶的磷酸化与多种疾病有关，包括癌症和糖尿病。 GSK3还由于其神经元Tau蛋白的磷酸化而与神经退行性疾病（例如阿尔茨海默氏病）相关。p70 S6 Kinase (p70S6K) is a member of the ribosomal S6 kinase family of Ser/Thr kinases. P70 S6K activity is controlled by multiple signaling pathways, including the MAPK, phosphoinositide-3 kinase (PI3K), and mTOR pathways. This regulation results in the phosphorylation of multiple sites of p70 S6K, including Thr389 within the linker domain, which is required for full activation. Activated p70 S6K phosphorylates several residues on the S6 ribosomal protein, thereby leading to increased protein synthesis.p70 S6激酶（p70S6K）是Ser / Thr激酶的核糖体S6激酶家族的成员。 P70 S6K活性受多种信号传导途径控制，包括MAPK，磷酸肌醇3激酶（PI3K）和mTOR途径。 该调节导致p70 S6K的多个位点（包括连接域内的Thr389）的磷酸化，这是完全激活所必需的。 活化的p70 S6K使S6核糖体蛋白上的多个残基磷酸化，从而导致蛋白质合成增加。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
QQ:1749072012