beta Catenin (Phospho) [pS45] Multispecies InstantOne ELISA Kit/多物种beta连环素（磷酸）[pS45] InstantOne ELISA试剂盒
货号：85-86142-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | beta Catenin |
物种（Tested） | 人，小鼠，大鼠 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Mouse) 12387,(Rat) 84353,(Human) 1499 |
| Gene symbol | Ctnnb1, Ctnnb1, CTNNB1 |
Gene aliases | Bfc, Catnb, Mesc, Catnb, CTNNB, MRD19, armadillo |
UniProt ID | (Mouse) Q02248,(Rat) Q9WU82,(Human) P35222 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and rat beta-Catenin (Ser45) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专为精确测量细胞裂解物中磷酸化的人，小鼠和大鼠β-连环蛋白（Ser45）而设计的。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.Beta-catenin是一种粘附连接（AJ）蛋白，最初被确定为细胞-细胞粘附结构的组成部分。 AJ通过调节细胞生长和细胞之间的粘附对于创建和维持上皮细胞层是必需的。 β-catenin与E-cadherin的胞质域相互作用，并将E-cadherin与α-catenin连接，后者又介导E-cadherin复合物锚定在皮质肌动蛋白细胞骨架上。研究表明，β-连环蛋白还与另一种含有腺瘤性息肉病大肠杆菌蛋白和微管的细胞骨架复合物结合，并与包括酪氨酸激酶，磷酸酶和Wnt / Wingless在内的多种信号通路相互作用。 β-连环蛋白，细胞骨架复合物和信号通路之间的相互作用可能调节形态发生。 β-catenin在几种毛囊细胞类型，基础和周围基质细胞以及内外根鞘的细胞中表达。 β-catenin的病理作用已在毛发恶性肿瘤（PTR），髓母细胞瘤（MDB），结直肠癌（CRC），卵巢癌和肿瘤发展中确定。在细胞核中，β-连环蛋白用于共同激活Lef / Tcf转录因子家族，该因子刺激靶基因的转录，包括编码促进细胞增殖的细胞周期蛋白D和c-myc的编码。对细胞增殖的影响是β-连环蛋白在肿瘤发生中作用的分子基础，特别是乳房，结肠，肝脏，肺，胃，前列腺和皮肤的实体瘤。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
beta Catenin (Phospho) [pS45] Multispecies InstantOne™ ELISA KitRepresentative data for beta Catenin (Phospho) [pS45] Multispecies InstantOne™ ELISA. A549 cells were either untreated (-) or treated with PMA/calyculin (+). Equal amounts of both untreated and treated cell lysates (5 µg) were analyzed at by either immunoblotting (left) or InstantOne™ ELISA (right). The intensity of the band at 94 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.β连环素（磷酸）[pS45] Multispecies InstantOne™ELISA的代表性数据。 A549细胞未经处理（-）或经PMA /钙蛋白（+）处理。 通过免疫印迹（左）或InstantOne™ELISA（右）分析等量未处理和已处理的细胞裂解液（5 µg）。 通过免疫印迹检测到的在94 kDa处的条带强度与InstantOne™ELISA吸收值相关。 |
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