IL-2 Human Uncoated ELISA Kit with Plates/人IL-2 ELISA试剂盒含未包被板
货号：88-7025-22,88-7025-86,88-7025-76
规格：2X96 Tests,10X96 Tests,20X96 Tests
价格：3125,6537,9393
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 2-250 pg/mL |
样本类型/体积 | 血浆 100 μL 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-2 |
| Protein aliases | Aldesleukin, IL-2, T-cell growth factor, TCGF, Interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL-2, IL2, lymphokine, TCGF |
| Gene ID | (Human) 3558 |
| Gene symbol | IL2 |
| UniProt ID | (Human) P60568 |
| The Human Interleukin-2 (IL-2) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of Humanmouse IL-2. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素2（IL-2）未包被的ELISA试剂盒包含预先配对的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量Humanmouse IL-2的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.白介素2（IL-2）是一种免疫调节性细胞因子，对于活化T细胞的增殖，B细胞，自然杀伤细胞，单核细胞和巨噬细胞的分化非常重要。 IL-2通过IL-2受体（IL-2R）发出信号，IL-2R是一种异源三聚体蛋白复合物，其γ链也被白介素4（IL-4）和白介素7（IL-7）共享。 IL-2基因在成熟胸腺细胞中的表达是单等位基因，代表了控制单个基因精确表达的异常调节模式。 在小鼠中类似于IL-2的基因的靶向破坏会导致溃疡性结肠炎样疾病，这提示该基因在对抗原刺激的免疫反应中起着至关重要的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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