| Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: This HIB19 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.PLoS pathogensActivation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.2016"25-0199 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses."Authors Tomić A,Varanasi PR,Golemac M,Malić S,Riese P,Borst EM,Mischak-Weissinger E,Guzmán CA,Krmpotić A,Jonjić S,Messerle M2.PloS oneExpression of Lectin-Like Transcript 1, the Ligand for CD161, in Rheumatoid Arthritis.2016"25-0199 was used in Flow cytometry/Cell sorting to investigate the presence and identity of LLT1-expressing cells in RA synovial fluid (SF) and synovial tissue (ST)."Authors Chalan P,Bijzet J,Huitema MG,Kroesen BJ,Brouwer E,Boots AM3.Mediators of inflammationDifferences of IL-1β Receptors Expression by Immunocompetent Cells Subsets in Rheumatoid Arthritis.2016"25-0199 was used in Flow cytometry/Cell sorting to study immune cell types and their differential expression of receptors to immunomodulatory cytokines in healthy and arthritic individuals."Authors Alshevskaya AA,Lopatnikova JA,Shkaruba NS,Chumasova OA,Sizikov AE,Karaulov AV,Kozlov VA,Sennikov SV4.Cellular and molecular immunologyRelationship between interleukin-1 type 1 and 2 receptor gene polymorphisms and the expression level of membrane-bound receptors.2015"25-0199 was used in Flow cytometry/Cell sorting to determine the relationship between interleukin-1 type 1 and type 2 receptor gene polymorphisms and the expression level of membrane-bound IL1-Rs.Authors Vasilyev FF,Silkov AN,Sennikov SV5.Nature medicineHomeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.2015"25-0199 was used in Flow cytometry/Cell sorting to demonstrate that exogenous PEPITEM reduces T cell recruitment into inflamed tissues in multiple animal models of autoimmune, inflammatory diseases."Authors Chimen M,McGettrick HM,Apta B,Kuravi SJ,Yates CM,Kennedy A,Odedra A,Alassiri M,Harrison M,Martin A,Barone F,Nayar S,Hitchcock JR,Cunningham AF,Raza K,Filer A,Copland DA,Dick AD,Robinson J,Kalia N,Walker LSK,Buckley CD,Nash GB,Narendran P,Rainger GE |
QQ:1749072012