ST2 (IL-33R) Mouse Uncoated ELISA Kit with Plates/小鼠ST2 (IL-33R) ELISA试剂盒含未包被板
货号：88-9334-22,88-9334-86,88-9334-76
规格：2X96 Tests,10X96 Tests,20X96 Tests
价格：3125,6537,9393
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 80 pg/mL |
| 检测范围 | 80-10,000 pg/mL |
样本类型/体积 | 血清 50 μL 上清液 50 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 包被缓冲液：10X PBS ELISA 包被缓冲液 - 检测稀释液：5X浓缩液 - 样品稀释液B: 12 mL of a 1X solution per plate - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) Solution - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-86） |
储存温度 | 2-8℃ |
| Protein name | ST2 |
| Protein aliases | Interleukin-1 receptor-like 1, Interleukin-33 receptor alpha chain, Lymphocyte antigen 84, Protein ST2, Protein T1 |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | DER4, Fit-1, Il1rl1, Ly84, St2, St2-rs1, ST2L, Ste2, T1, T1/ST2 |
| Gene ID | (Mouse) 17082 |
| Gene symbol | Il1rl1 |
| UniProt ID | (Mouse) P14719 |
| The Mouse ST-2 (IL-33R) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-33R. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠ST-2（IL-33R）未包被ELISA试剂盒包含预匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠IL-33R的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息ST2 is a member of the interleukin 1 receptor family. Studies of ST2 in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells.ST2 belongs to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain. ST2 is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Reports also suggest its possible role in helper T-cell function as well as in cell growth. Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form. ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease. Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.ST2是白介素1受体家族的成员。小鼠ST2的研究表明，该受体可被促炎性刺激诱导，并可能参与辅助性T细胞的功能。 ST2属于白介素-1受体家族，具有三个Ig样C2型（免疫球蛋白样）域和一个TIR域。 ST2是白介素33（IL-33）的受体，经刺激后募集信号分子MYD88，IRAK1，IRAK4和TRAF6，随后磷酸化MAPK3 / ERK1和/或MAPK1 / ERK2，MAPK14和MAPK8。报告还表明其可能在辅助T细胞功能以及细胞生长中的作用。已经报道了ST2的三种同工型，其中同工型C是膜形式，而同工型B是分泌形式。 ST2可以由促炎性刺激诱导，也可以在自身免疫性疾病的血清中发现。不同的同工型仅限于不同的组织，而该蛋白在肾脏，肺，胎盘，胃，骨骼肌，结肠和小肠中的高表达是明显的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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