Ig Isotyping Rat Uncoated ELISA Kit/大鼠Ig同型鉴定ELISA试剂盒
货号：88-50640-88
规格：10 x 96 tests
价格：6797
产品类型：ELISA检测
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：IgG
荧光染料：其它
| The Rat Immunoglobulin(Ig) Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing enzyme linked immunosorbent assays (ELISA) to detect and identify rat Igs Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 组成成分： |
| - Capture Antibody- Detection Antibody- Standard- 10X Coating Buffer- 5X ELISA/ELISPOT Diluent- Substrate- Certificate of Analysis |
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