IL-33 Mouse Uncoated ELISA Kit with Plates/小鼠IL-33 ELISA试剂盒含未包被板
货号：88-7333-86,88-7333-22
规格：10X96 Tests,2X96 Tests
价格：6537,3125
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 25 pg/mL |
| 检测范围 | 25-3,000 pg/mL |
样本类型/体积 | 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10XPBS包被缓冲液：用于包被捕获抗体的缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Streptavidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) Substrate Solution - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：NUNC Maxisorp flat-bottom（含产品目录号，后缀 -22，-44，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-33 |
| Protein aliases | IL-33, Interleukin-33, Interleukin-33(102-266), Interleukin-33(109-266), nuclear factor from high endothelial venules |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | 9230117N10Rik, Il-33, Il1f11, Il33, NF-HEV |
| Gene ID | (Mouse) 77125 |
| Gene symbol | Il33 |
| UniProt ID | (Mouse) Q8BVZ5 |
| The Mouse Interleukin 33 (IL-33) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-33. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素33（IL-33）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠IL-33的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.IL-33（Interleukin-33）是一种270个氨基酸的高分化蛋白，属于具有IL-1样C末端结构域的IL-1家族。 IL-33是一种双重功能蛋白，可以同时充当促炎细胞因子和具有转录调控特性的细胞内核因子。 IL-33与IL1RL1 / ST2结合并通过IL1RL1 / ST2发出信号，其刺激募集MYD88，IRAK1，IRAK4和TRAF6。 IL-33激活NF-κB和MAP激酶，并驱动体外极化的TH2细胞产生TH2相关的细胞因子。在体内，IL-33诱导IL-4，IL-5和IL-13的表达，并导致粘膜器官发生严重的病理变化。 IL-33被CASP1蛋白水解转化为成熟形式，并在扁桃体，派伊尔氏淋巴结和肠系膜淋巴结中发现的高内皮微静脉中高度表达，并且在胎盘中几乎无法检测到。长期对小鼠进行IL-33治疗会导致嗜酸性粒细胞增多，脾肿大和粘膜器官（如肺，食道和小肠）的严重病理变化。最近的实验表明，IL-33还可以与异染色质共定位，并具有转录抑制活性，这表明IL-33既可以作为促炎细胞因子，又可以作为具有转录调控特性的细胞内核因子。尽管具有预期的分子量，但IL-33在SDS-PAGE中通常会以较高的分子量运行。研究表明，IL-33还可以与异染色质共定位，并具有转录抑制因子活性，这表明IL-33既可以充当促炎细胞因子，又可以具有转录调控特性的细胞内核因子。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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