IL-13 Mouse Uncoated ELISA Kit/小鼠IL-13未包被ELISA试剂盒
货号：88-7137-88,88-7137-77
规格：10X96 Tests,20X96 Tests
价格：6261,8846
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 4 pg/mL |
| 检测范围 | 4-500 pg/mL |
样本类型/体积 | 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 包被缓冲液：用于包被捕获抗体的缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物容易我：Tetramethylbenzidine (TMB) Substrate Solution - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-44，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-13 |
| Protein aliases | IL-13, T-cell activation protein P600, Interleukin-13 |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Il-13, Il13 |
| Gene ID | (Mouse) 16163 |
| Gene symbol | Il13 |
| UniProt ID | (Mouse) P20109 |
| The Mouse Interleukin 13 (IL-13) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-13. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素13（IL-13）未包被的ELISA试剂盒包含预先匹配的抗体对，以及用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠IL-13的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.IL-13是主要由活化的Th2细胞产生的免疫调节细胞因子，参与调节炎症和免疫反应。 IL-13下调巨噬细胞活性，从而抑制促炎性细胞因子和趋化因子的产生，并通过独立于IgE和嗜酸性粒细胞的机制参与过敏原诱发的哮喘。 IL-13还调节B细胞成熟和分化的几个阶段，上调CD23和MHC II类表达，并促进B细胞的IgE同种型转换。 IL-13在结构上与IL-3，IL-4，IL-5和GM-CSF非常相似。 编码IL-13，IL3，IL5，IL4和CSF2的基因在5q染色体上形成细胞因子基因簇，其中IL-13基因特别接近IL4。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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