IL-15 Human Uncoated ELISA Kit/人IL-15未包被ELISA试剂盒
货号：88-7620-88
规格：10X96 Tests
价格：6261
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 20 pg/mL |
| 检测范围 | 20-2,500 pg/mL |
样本类型/体积 | 血清 100 μL 血浆 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-15 |
| Protein aliases | IL-15, Interleukin-15 |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL-15, IL15 |
| Gene ID | (Human) 3600 |
| Gene symbol | IL15 |
| UniProt ID | (Human) P40933 |
| The Human Interleukin 15 (IL-15) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-15. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素15（IL-15）未包被的ELISA试剂盒包含预先匹配的抗体对，以及用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量人IL-15的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.IL-15（白介素15）是一种调节T和自然杀伤细胞活化和增殖的细胞因子。 IL-15和IL-2具有许多生物学活性，因为它们都结合了常见的造血素受体亚基，并且可能竞争同一个受体，从而对彼此的活性产生负面调节。 CD8 +存储单元的数量显示受IL-15和IL-2之间的平衡控制。 IL-15诱导JAK激酶的激活，以及转录激活因子STAT3，STAT5和STAT6的磷酸化和激活。在小鼠中，研究表明IL-15可能通过STAT6的转录激活活性来增加凋亡抑制剂Bcl-xL的表达，从而阻止凋亡。 IL-15在T和B淋巴细胞，自然杀伤细胞，巨噬细胞和单核细胞的生长和分化以及许多重要的细胞内信号分子的激活中起着重要作用。因此，IL-15对于免疫反应，同种异体移植排斥和自身免疫性疾病的发病机制可能是必不可少的。此外，IL-15是广泛表达的促炎性细胞因子，并且已经显示出它在几种炎性疾病中起作用，包括类风湿性关节炎，牛皮癣和肺炎性疾病。新兴数据表明，IL-15可以作为良好的治疗靶标，因为在牛皮癣和糖尿病模型中，IL-15中和作用似乎是有益的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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