IL-21 Human Uncoated ELISA Kit/人IL-21未包被ELISA试剂盒
货号：88-8218-88,88-8218-77
规格：10X96 Tests,20X96 Tests
价格：6261,8846
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 8 pg/mL |
| 检测范围 | 8-1,000 pg/mL |
样本类型/体积 | 血清 100 μL 血浆 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -包被缓冲液：10X PBS ELISA包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB)溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-21 |
| Protein aliases | IL-21, Za11, Interleukin-21, interleukin-21 isoform |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CVID11, IL-21, IL21, Za11 |
| Gene ID | (Human) 59067 |
| Gene symbol | IL21 |
| UniProt ID | (Human) Q9HBE4 |
| The Human Interleukin 21 (IL-21) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-21. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素21（IL-21）未包被ELISA试剂盒包含预先匹配的抗体对，以及用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量人IL-21的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.IL-21是一种17 kDa的免疫调节细胞因子，主要由自然杀伤细胞（NKT），T辅助细胞（Th）17和T滤泡辅助细胞（TFH）细胞产生。在TFH细胞中，IL-21表达导致通过IL-21受体（IL-21R）和STAT3的自分泌信号传导，从而导致Bcl6进行额外的转录激活。与Th1细胞的IFNγ和Th2细胞的IL-4一样，IL-21对于TFH细胞发育和效应子功能至关重要。 IL-21在依赖T细胞的B细胞分化为浆细胞和记忆细胞，刺激IgG产生和诱导天然B细胞凋亡信号传导中发挥作用。在Th17细胞中，IL-21的表达和通过STAT3，IRF4和RORγt引起的自分泌反馈导致IL-23R上调，从而为Th17细胞做好准备，使其通过炎性细胞因子IL-23进行成熟和维持。 IL-21在上调IRF4和RORγt的同时，还介导Foxp3的下调。 IL-21缺陷小鼠由于无法上调与Th17相关的分子而受到化学治疗，免受发展性结肠炎的侵害。相比之下，化学诱导的结肠炎小鼠模型中存在IL-21水平升高。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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