IL-10 Mouse Uncoated ELISA Kit with Plates/小鼠IL-10 ELISA试剂盒含未包被板

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IL-10 Mouse Uncoated ELISA Kit with Plates/小鼠IL-10 ELISA试剂盒含未包被板

货号:88-7105-86,88-7105-76,88-7105-22

规格:10X96 Tests,20X96 Tests,2X96 Tests

价格:6537,9393,3125

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

32 pg/mL

检测范围32-4,000 pg/mL

样本类型/体积

血清 100 μL

上清液 100 μL

操作时间

1 hr 45 min

测试时间

24 hr 30 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10X 96 Tests

组份

- 捕获抗体:Pre-titrated, 纯化抗体

- 检测抗体:Pre-titrated, Biotin偶联抗体

- 标准品:重组细胞因子,用于生成标准曲线和校准样品

-10XPBS包被缓冲液:用于包被捕获抗体的缓冲液

- 检测稀释液:5X浓缩液

- 检测酶:Pre-titrated Avidin-HRP

- 底物溶液:Tetramethylbenzidine (TMB) Substrate Solution

- 分析证书:特定批次说明书用于抗体和标准品稀释

- 96孔板:Corning Costar 9018(含产品目录号,后缀 -22,-44,-76,-86)

储存温度

2-8℃

Protein nameIL-10
Protein aliasesCSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10

物种(Tested)

小鼠

试剂盒类型

Uncoated ELISA Kitwith Plates

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCSIF, Il-10, Il10
Gene ID(Mouse) 16153
Gene symbolIl10
UniProt ID(Mouse) P18893
The Mouse Interleukin 10 (IL-10) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-10. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素10(IL-10)未包被的ELISA试剂盒包含预先匹配的抗体对,板和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量小鼠IL-10的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.白细胞介素10是一种多效细胞因子,在调节淋巴样和髓样细胞功能中起着重要作用。在人类中,IL-10由活化的CD8(+)外周血T细胞,T辅助CD4(+)T细胞克隆产生。由于IL-10具有阻断细胞因子合成的能力和巨噬细胞的几种辅助细胞功能,因此该细胞因子是巨噬细胞,T细胞和NK细胞的效应子功能的有效抑制剂。另外,IL-10参与调节B细胞,肥大细胞和胸腺细胞的增殖和分化。已经通过克隆编码细胞因子的cDNA来确定人IL-10的一级结构。相应的蛋白质具有160个氨基酸,预测分子量为18.5 kDa。根据其主要结构,IL-10是细胞因子的四个a-螺旋束家族的成员。在溶液中,人IL-10是表观分子量为39kDa的同型二聚体。尽管它包含一个N连接的糖基化位点,但它缺乏可检测的碳水化合物。因此,在大肠杆菌中表达的重组蛋白保留了所有已知的生物学活性。人IL-10基因位于1号染色体上,在基因组中以单拷贝形式存在。人IL-10与鼠类IL-10具有很强的DNA和氨基酸序列同源性,在爱泼斯坦-巴尔病毒基因组BCRF1中具有开放阅读框,它具有许多细胞因子的生物活性,因此可能在细胞因子中起一定作用。主机与病毒的交互。 IL-10的免疫抑制特性表明IL-10在抑制器官移植后移植物排斥反应方面的临床应用可能。 IL-10还可以发挥强大的抗炎活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。
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