IL-4 Mouse Uncoated ELISA Kit with Plates/小鼠IL-4 ELISA试剂盒含未包被板
货号：88-7044-86,88-7044-76,88-7044-22
规格：10X96 Tests,20X96 Tests,2X96 Tests
价格：6537,9393,2856
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 4pg/mL |
| 检测范围 | 4-500 pg/mL |
样本类型/体积 | 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品： 重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体 -5X ELISA/ELISPOT稀释液：用于稀释检测抗体和酶的缓冲液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：1X TMB底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀-22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-4 |
| Protein aliases | B-cell growth factor 1, B-cell IgG differentiation factor, B-cell stimulatory factor 1, BSF-1, IGG1 induction factor, IL-4, Interleukin-4, Lymphocyte stimulatory factor 1 |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Mouse) 16189 |
| Gene aliases | BSF-1, Il-4, Il4 |
| Gene symbol | Il4 |
UniProt ID | (Mouse) P07750 |
| The Mouse Interleukin-4 (IL-4) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-4. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素4（IL-4）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠IL-4的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.白介素-4（IL-4）是由活化的T细胞产生的多效性免疫调节细胞因子，可作为白介素4受体的配体。 已经发现IL-4具有最广泛的作用范围。 白介素4受体也与IL13结合，这可能有助于该细胞因子和IL13的许多重叠功能。 STAT6是一种信号转导和转录激活因子，已显示在介导IL-4的免疫调节信号中起重要作用。 IL-4基因，IL-3，IL-5，IL-13和CSF2在5q号染色体上形成细胞因子基因簇。 特别地，IL-4在染色体上紧邻IL13。 此外，发现IL-4基因，IL13和IL5由染色体上120kb范围内的几个远程调控元件协调。 IL-4δ2是IL-4的已知剪接变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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