IL-6 Human Uncoated ELISA Kit with Plates/人IL-6 ELISA试剂盒含未包被板
货号：88-7066-22,88-7066-86,88-7066-76
规格：2X96 Tests,10X96 Tests,20X96 Tests
价格：2856,6537,9393
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2pg/mL |
| 检测范围 | 2-200 pg/mL |
样本类型/体积 | 血清 100 μL 上清液 100 μL 血浆 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品： 重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体 - 5X ELISA/ELISPOT稀释液：用于稀释检测抗体和酶的缓冲液 - 检测酶：Pre-titrated Streptavidin-HRP Concentrate (100X) - 底物溶液：1X TMB 底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀-22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-6 |
| Protein aliases | B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2, interleukin BSF-2, Interleukin-6 |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Human) 3569 |
| Gene aliases | BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6, IL6 |
| Gene symbol | IL6 |
UniProt ID | (Human) P05231 |
| The Human Interleukin-6 (IL-6) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-6. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.白介素6（IL-6）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量人IL-6的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.白介素6（IL-6）是一种多功能的26 kD蛋白，最初是在RNA刺激的成纤维细胞样细胞的培养基中发现的。 IL-6似乎直接参与感染和细胞损伤后发生的反应，它可能被证明在调节急性期反应中作为IL-1和TNF-a很重要。据报道IL-6由成纤维细胞，活化的T细胞，活化的单核细胞或巨噬细胞和内皮细胞产生。 IL-6作用于多种细胞，包括骨髓祖细胞，T细胞，B细胞和肝细胞。另外，IL-6似乎在T淋巴细胞的增殖中与IL-2相互作用。 IL-6增强了IL-3对多能造血祖细胞的增殖作用。 IL-6在B细胞向浆细胞的分化中起关键作用，并且是浆细胞瘤和骨髓瘤的有效生长因子。 IL-6是一种非常有用的培养补充剂，可用于产生大量产生抗体的杂交瘤。 IL-6主要在急性和慢性炎症部位产生，被分泌到血清中并通过白介素6受体α诱导转录炎症反应。 IL-6的功能涉及多种与炎症相关的疾病状态，包括糖尿病和全身性青少年类风湿性关节炎。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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