IL-17AF (heterodimer) Human Uncoated ELISA Kit/人IL-17AF (heterodimer)未包被ELISA试剂盒
货号：88-7117-88
规格：10 x 96 tests
价格：6261
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30 pg/mL |
| 检测范围 | 30-4,000 pg/mL |
样本类型/体积 | 血清 100 μL 血浆 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated,biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 酶：pre-titrated Streptavidin-HRP - 底物：Tetramethylbenzidine (TMB)溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-17AF |
| Protein aliases | CTLA-8, Cytokine ML-1, Cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated protein 8, IL-17, IL-17A, IL-17F, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), Interleukin-17A, Interleukin-17F |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CANDF6, CTLA-8, CTLA8, IL-17, IL-17A, IL-17F, IL17, IL17A, IL17F, ML-1, ML1 |
| Gene ID | (Human) 3605,(Human) 112744 |
| Gene symbol | IL17A, IL17F |
| UniProt ID | (Human) Q16552,(Human) Q96PD4 |
| The Human IL-17AF (heterodimer) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-17AF. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人IL-17AF（异二聚体）未包被ELISA试剂盒包含预先匹配的抗体对，以及用于进行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量人IL-17AF的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17) and to CTLA-8.This recombinant protein is E. coli expressed. Insect cell expressed Human IL-17A Recombinant Protein (cat. 14-8180) is also available.白细胞介素17A（IL-17A）是一种CD4 + T细胞衍生的细胞因子，可促进细胞系中的炎症反应，并在类风湿性关节炎，哮喘，多发性硬化症，牛皮癣和移植排斥反应中升高。从CD4 + T细胞文库中分离出编码人IL-17A的cDNA。编码的蛋白质与HVS13（来自T淋巴性疱疹病毒saimiri的开放阅读框）具有72％的氨基酸同一性，与小鼠CTLA-8（细胞毒性T淋巴细胞相关抗原8）具有63％的氨基酸同一性。人IL-17A以糖基化的20-30 kD同型二聚体形式存在。活化的外周血CD4 + T细胞可产生高水平的IL-17A同型二聚体。 IL-17A增强人成纤维细胞中细胞内粘附分子1（ICAM-1）的表达。人IL-17A还刺激上皮，内皮或成纤维细胞分泌IL-6，IL-8，G-CSF和PGE2。在人IL-17A存在的情况下，成纤维细胞可以维持CD34 +造血祖细胞的增殖并诱导成熟为嗜中性粒细胞。小鼠，大鼠和人IL-17A可以诱导小鼠基质细胞中的IL-6分泌，这表明所有同源物都可以识别小鼠受体。 IL-17A与受体结合，该受体也与HVS13（病毒IL-17）和CTLA-8结合。这种重组蛋白在大肠杆菌中表达。也可以使用昆虫细胞表达的人IL-17A重组蛋白（目录14-8180）。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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