5'-DMT-A(PAC) Synthesis Column; 1000 Å (RNA)
货号：CG1-1000R-1,CG1-1000R-2
规格：1 µmol,200 nmol
价格：112.56,84.42
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| This synthesis column, packed with 5'-DMT-A(PAC)-Suc-RNA CPG, is used for the placement of A on the 3' end of oligoribonucleotides.This support has a 2’ acetyl protecting group which is easily cleaved during deprotection.The 1000 Å CPG is best suited for the synthesis of RNA sequences up to 100-mers in length.The use of this 5’-DMT protected RNA support is identical to a DNA support with the same protection groups. |
| 特性： |
| Appearance: white solidExtinction Coefficient at 260 nm: 15200 |
| 产品用途： |
| Synthesis conditions: If using PAC protected supports or reagents we recommend using phenoxyacetic anhydride or t.butyl phenoacetal acid anhydride(FAST deprotecting capping) as the CAP A reagent. Standard acetic anhydride (CAP A) can cause acetylation of the PAC protected primary amines during synthesis.Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites. We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase. Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 329.21 |
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