Iso-Insulin Human Instant ELISA Kit/人Iso-胰岛素一步法ELISA试剂盒
货号：BMS2003INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 16.6 pg/mL | 产品规格： | 128 tests |
检测范围： | 31.3-2,000 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | Insulin |
测试时间： | 1 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 5% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 4.5% | 检测抗体偶联物： | HRP |
| 同名基因： | IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10 | 基因名称： | INS |
样品类型/体积： | 血清 50 µL 血浆 50µL 上清液 50µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Iso Insulin ELISA quantitates Hu Iso Insulin in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu Iso Insulin.人异胰岛素ELISA可以定量测定人血清，血浆，细胞培养上清液或其他体液中的胡异胰岛素。 该检测试剂盒将专门识别天然和重组人胡索胰岛素。Principle of the method 方法原理The Human Iso Insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人胰岛素夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Insulin is a key player in the control of carbohydrate metabolism. It facilitates the entry of glucose into muscle, adipose and several oher tissues. Insulin is composed of two chains referred to as A-chain (21 amino acids) and B-chain (30 amino acids) which are connected by two disulfide bonds, with an additional disulfide bond within the A-chain. The Insulin synthesis takes place in the beta-cells of the islets of Langerhans in the pancreas. Insulin secretion is mainly caused by elevated glucose concentration of plasma. Proinsulin is cleaved into Insulin and C-peptide, which are then secreed to circulation in equimolecular amounts. Very low levels of Insulin are observed with diabetes mellitus type I, and hypopituitarism. Diabetes mellitus type II, obesity, insulinoma, cushings syndrome and acromegaly show significantly raised Insulin levels.胰岛素是控制碳水化合物代谢的关键因素。 它有助于葡萄糖进入肌肉，脂肪和一些其他组织。 胰岛素由称为A链（21个氨基酸）和B链（30个氨基酸）的两条链组成，它们通过两个二硫键连接，并且在A链内还有一个二硫键。 胰岛素的合成发生在胰脏朗格汉斯胰岛的β细胞中。 胰岛素分泌主要由血浆葡萄糖浓度升高引起。 胰岛素原被切割成胰岛素和C肽，然后以等分子量被分泌到循环中。 I型糖尿病和垂体功能低下症患者的胰岛素水平非常低。 II型糖尿病，肥胖症，胰岛素瘤，库欣综合征和肢端肥大症显示胰岛素水平明显升高。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
Iso-Insulin Human Instant ELISA™ KitRepresentative standard curve for Human Iso-Insulin Instant ELISA |
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