C6 CPG Synthesis Column (DMT-1,6-Hexanediol-Glyc-CPG); 1000 Å
货号：CG1-5013-1,CG1-5013-2,CG1-5013-5
规格：1 µmol,200 nmol,50 nmol
价格：211.05,84.42,56.28
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| C6 Spacer CPG allows for a six carbon spacer at the 3' end of an oligonucleotide. The 3' spacer inactivates the 3' OH towards enzymatic processing and may be used in lieu of 3' phosphate. The 1000 Å pore size is best suited for the synthesis of DNA sequences up to 100-mers in length. |
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| ▪Appearance:White Powder |
| 产品用途： |
| Cleavage conditions:The glycolate spacer is more readily cleaved than the usual succinyl linkage. Cleave for 30-minutes at room temperature with concentrated NH4OH or 15 minutes at 60°C.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:180.14 |
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