IL-5 Human Instant ELISA Kit/人IL-5一步法ELISA试剂盒
货号：BMS278INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 1.45 pg/mL | 产品规格： | 128 tests |
检测范围： | 7.8-500 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | IL-5 |
测试时间： | 3 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 10.4% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 9.6% | 检测抗体偶联物： | Biotin |
| 同名基因： | EDF, IL-5, TRF | 基因名称： | IL5 |
样品类型/体积： | 血清 50 µL 血浆 50µL 上清液 50µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Interleukin 5 (Hu IL-5) ELISA quantitates Hu IL-5 in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-5.人白介素5（Hu IL-5）ELISA定量了人血清，血浆，细胞培养上清液或其他体液中的Hu IL-5。 该检测试剂盒将专门识别天然和重组的Hu IL-5。Principle of the method 方法原理The Human IL-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-5夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.IL-5是一种细胞因子，可同时充当B细胞和嗜酸性粒细胞的生长和分化因子。 IL-5细胞因子是嗜曙红细胞生成，嗜酸性粒细胞成熟和激活的主要调节因子。 据报道，这种细胞因子的产生增加与哮喘或嗜酸性粒细胞增多症有关。 IL-5细胞因子的受体是异二聚体，其β亚基与白细胞介素3（IL3）和集落刺激因子2（CSF2 / GM-CSF）的受体共享。 IL-5与白介素4（IL4），白介素13（IL13）和CSF2一起，在5号染色体上形成细胞因子基因簇。发现IL-5，IL4和IL13受长距离协同调节。 调控元件在5q31号染色体上分布了120千个碱基。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
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