2'-O-Methyl-U-Suc-CPG; 500 Å (RNA) Synthesis Column
货号:CG5-1300MR-1,CG5-1300MR-2
规格:1 µmol,200 nmol
价格:253.26,211.05
产品类型:核酸合成
品牌:LGC Biosearch Technologies
2-O’-Methyl-U-Suc-CPG is used for synthesis of oligonucleotides that contain nuclease resistant 2’-O-methyl linkages to prevent degradation. The 2’-O-Methyl-U-Suc-CPG requires no special handling or deprotection reagents. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length. |
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Synthesis conditions:Follow the instrument manufacturer's protocol when using this product.Cleavage conditions:Use concentrated ammonia for 90 minutes at 25°C or for 30 minutes at 60°C.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
This RNA support is compatible with deprotection chemistries used with 2’ Fluoro; 2' modified phosphoramidites and TBDMS protected reagents.AMA is not recommended for sequences containing uridine bases. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:320.19 |
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