Reaction Buffer for phi29 DNA Polymerase (10X)

货号：B62

规格：5 x 1.0 mL

价格：244

产品类型：酶及抑制剂

品牌：Thermo Fisher

描述Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi 29 DNA polymerase.Unbiased amplification of human genomic DNA with phi29 DNA PolymeraseUnbiased amplification of human genomic DNA with phi29 DNA Polymerase.100 copies (0.33ng) of human genomic DNA were amplified with phi29 DNA Polymerase. Radioactive [α-33P]-dATP incorporation into the DE-81 adsorbable form was calculated to quantify amplification of total DNA. Amplification efficiency of specific genes located in different chromosomes was determined by real-time PCR using the ABI 7700.

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