| The ProQuantum MouseTNF alphaImmunoassay Kit is designed to provide highly sensitive quantitative measurements of mouseTNF alpha protein in small sample volumes. Utilizing proximity ligation assay (PLA) technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.ProQuantum 免疫检测试剂盒进行少量小鼠TNF alpha蛋白的高灵敏度定量检测。 利用邻近连接测定（PLA）技术，该测定将高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能结合在一起，从而实现了一个简单而强大的下一代蛋白质定量平台。 友好的工作流程与直观的基于云的分析软件相结合，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology.ProQuantum免疫测定法使用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。 在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后创建模板链进行扩增。 该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和宽动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up customized assay instructions, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。 每个试剂盒中总共有7个组件。 首先，混合抗体-结合物物，稀释样品，并在工作板上创建标准曲线。 然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。 合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。 运行完成后，将结果文件导入https://apps.thermofisher.com/apps/proquantum的基于ProQuantum云的软件中。 使用此软件，可以轻松分析数据以获得蛋白质浓度值。 该软件允许您设置标准曲线，设计板布局，设置定制化验说明并获得可靠的逐组统计比较。Target information 靶标信息TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.TNFα是一种多功能促炎细胞因子，属于肿瘤坏死因子（TNF）超家族。这种细胞因子主要由巨噬细胞分泌，并与其受体TNFRSF1A / TNFR1和TNFRSF1B / TNFBR结合。 TNFα参与免疫细胞的调节，细胞增殖，分化，凋亡，脂质代谢和凝血。 TNFα以两个，三个或五个非共价连接的单元的多聚体形式存在，但在非还原条件下在SDS-PAGE后显示一个17 kDa的条带。小鼠的基因敲除研究还提示了TNFα的神经保护功能，并且已经观察到当注射到荷瘤小鼠中时会引起肿瘤坏死。 TNF-α的其他功能包括其在对细菌，病毒，寄生虫和某些真菌感染的免疫反应中的作用，以及在特定肿瘤坏死中的作用。 TNFα引起某些转化细胞的细胞溶解或细胞停滞，与干扰素-γ的细胞毒性具有协同作用。该细胞因子与多种疾病有关，包括自身免疫性疾病，胰岛素抵抗和癌症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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