Mouse anti-Rat IgG (H+L) Secondary Antibody, Biotin/小鼠抗大鼠IgG (H+L)二抗, Biotin

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Mouse anti-Rat IgG (H+L) Secondary Antibody, Biotin/小鼠抗大鼠IgG (H+L)二抗, Biotin

货号:13-4813-85

规格:500μg

价格:1283

产品类型:荧光二抗

品牌:Thermo Fisher

物种:大鼠

宿主:小鼠

抗体亚型:IgG

荧光染料:Biotin

类型:多抗同型对照:IgG
浓度:0.5 mg/mL用法:

Assay-Dependent(ELISA);0.25 µg/test(Flow);Assay-Dependent(IHC (F));Assay-Dependent(WB)
该抗体将识别其他大鼠免疫球蛋白的轻链和重链。尚未检测到对非免疫球蛋白大鼠血清蛋白的反应。报告的应用:与酶或荧光标记的抗生蛋白链菌素(SAV)或抗生物素蛋白(AV)结合使用,该多克隆抗体可用于ELISA,免疫印迹(WB),免疫组化染色和流式细胞仪检测纯化的大鼠免疫球蛋白(IgG)。分析。测试的应用程序:生物素抗大鼠IgG已通过流式细胞仪分析进行测试,以检测纯化的大鼠单克隆抗体。每次测试可使用小于或等于0.25 µg的水。测试的定义是将以100 µL的最终体积对细胞样品染色的抗体量(µg)。细胞数应根据经验确定,但范围为10 ^ 5至10 ^ 8个细胞/测试。建议在目标分析中仔细滴定抗体以获得最佳性能。此外,它已经通过ELISA进行了吸附和测试,以确保与人,牛,马和小鼠血清蛋白的交叉反应最少,但可能与其他物种的免疫球蛋白发生交叉反应。过滤:制造后0.2 µm过滤。靶标信息抗大鼠第二抗体是具有亲和力的抗体,对大鼠免疫球蛋白具有良好的特异性,可用于检测,分选或纯化其特定靶标。二抗提供了更多的多功能性,使用户能够使用许多检测系统(例如HRP,AP,荧光)。它们还可以通过信号放大来提供更高的灵敏度,因为多个二级抗体可以与单个一级抗体结合。最常见地,通过用来自靶物种的免疫球蛋白的汇集群体对宿主动物进行免疫来产生二抗,并且可以进一步纯化和修饰(即免疫亲和层析,抗体片段化,标记缀合等)以产生高度特异性的试剂。

推荐产品:

二抗

荧光二抗

免疫组化一抗

标签抗体

参考文献:

1.Nature communicationsThe persistence of low-grade inflammatory monocytes contributes to aggravated atherosclerosis."13-4813 was used in Immunofluorescence to demonstrate that subclinical endotoxemia aggravates murine atherosclerosis through programming monocytes into a non-resolving inflammatory state."AuthorsGeng S,Chen K,Yuan R,Peng L,Maitra U,Diao N,Chen C,Zhang Y,Hu Y,Qi CF,Pierce S,Ling W,Xiong H,Li L2.Cell metabolismThe small molecule harmine is an antidiabetic cell-type-specific regulator of PPARgamma expression."13-4813 was used in Immunohistochemistry to identify harmine as an antidiabetic cell-type-specific regulator of PPARgamma expression."AuthorsWaki H,Park KW,Mitro N,Pei L,Damoiseaux R,Wilpitz DC,Reue K,Saez E,Tontonoz P3.Nature communicationsThe persistence of low-grade inflammatory monocytes contributes to aggravated atherosclerosis."13-4813 was used in Immunofluorescence to demonstrate that subclinical endotoxemia aggravates murine atherosclerosis through programming monocytes into a non-resolving inflammatory state."AuthorsGeng S,Chen K,Yuan R,Peng L,Maitra U,Diao N,Chen C,Zhang Y,Hu Y,Qi CF,Pierce S,Ling W,Xiong H,Li L4.Molecular therapy : the journal of the American Society of Gene TherapyAAV6-mediated systemic shRNA delivery reverses disease in a mouse model of facioscapulohumeral muscular dystrophy."13-4813 was used in Immunohistochemistry to show that it is possible to reverse dominant muscle disease in a mouse model of facioscapulohumeral muscular dystrophy."AuthorsBortolanza S,Nonis A,Sanvito F,Maciotta S,Sitia G,Wei J,Torrente Y,Di Serio C,Chamberlain JR,Gabellini D5.Pigment cell and melanoma researchImmune responses in a mouse model of vitiligo with spontaneous epidermal de- and repigmentation."13-4813 was used in Immunohistochemistry to demonstrate attenuation of acute rejection following local immunosuppression with a single subtherapeutic dose of methylprednisolone delivered as a liver-targeted prodrug."AuthorsEby JM,Kang HK,Klarquist J,Chatterjee S,Mosenson JA,Nishimura MI,Garrett-Mayer E,Longley BJ,Engelhard VH,Mehrotra S,Le Poole IC
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