Quasar 705 CPG Super Column; 500 Å
货号：SCG5-5067-1,SCG5-5067-2
规格：1 µmol,200 nmol
价格：1266.3,393.96
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Quasar® 705 is an indocarbocyanine which fluoresces in the red region of the visible spectrum. This compound is a direct replacement for Cy™5.5. This column is packed with Quasar 705 CPG which is used for the 3' labeling of fluorogenic probes used in 5' nuclease assays and other detection assays. BHQ-2 will quench the Quasar 705 moiety. |
| 特性： |
| ▪ Formula:C72H80N4O8▪ Molecular Weight:1129.43▪ Appearance:blue powder▪ Absorption Maximum (Lambda Max):691▪ Extinction Coefficient at Lambda max:211000▪ Extinction Coefficient at 260 nm:17000▪ Fluorescence Maximum:709 |
| 产品用途： |
| Synthesis conditions:Follow the instrument manufacturer's protocol, with noted changes, when using this product.Cleavage conditions:Use concentrated NH4OH for 45 minutes at 25° C.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated NH4OH for 5 hours at 25°C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated NH4OH for 12 hours at room temperature. Some degradation of the Q705 may occur.We suggest using fast deprotecting amidites for DNA synthesis on this support.
Dye conjugates are light sensitive and must be protected from unnecessary exposure. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is：732.87 |
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