Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, FITC/大鼠抗小鼠IgG1（重链）二抗，FITC

货号：MA5-16785

规格：500 µg

价格：5979

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Purified murine IgG1 from BALB/c mice

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：LO-MG1-2

荧光染料：FITC

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	5 µg/mL(Flow)；1 mg/mL(ICC)；1 mg/mL(IF)

产品详细信息For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.Rat anti Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2 recognizes murine IgG1, and does not bind other mouse immunoglobulin classes or subclasses.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG1 (Heavy chain) Secondary Antibody (MA5-16785) in IFImmunofluorescence analysis of Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, FITC conjugate was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 hours at room temperature. Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, FITC conjugate (Product # MA5-16785) was used at a concentration of 0.1µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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