IL-28A Human Uncoated ELISA Kit with Plates/人IL-28A ELISA试剂盒含未包被板
货号：88-52102-22
规格：2 x 96 tests
价格：3125
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15.6 pg/mL |
| 检测范围 | 15.6-1,000 pg/mL |
样本类型/体积 | 血清 50 μL 血浆 50 μL 上清液 50 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated,biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-28A |
| Protein aliases | Cytokine Zcyto20, IFN-lambda-2, Interleukin-28A, IL-28A, Interferon lambda-2, interferon, lambda 2, interferon-lambda 2, interleukin 28A (interferon, lambda 2) |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IFNL2, IL-28A, IL28A, ZCYTO20 |
| Gene ID | (Human) 282616 |
| Gene symbol | IFNL2 |
| UniProt ID | (Human) Q8IZJ0 |
The Human Interleukin 28A (IL-28A) Uncoated ELISA Kit contains pre-matched antibody pairs ,plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-28A. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素28A（IL-28A）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量人IL-28A的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role.IL-28A属于IFN lambda家族，是IL-10超家族中的一种新型细胞因子家族。该家族的三个成员是IL-29（IFN lambda 1），IL-28A（IFN lambda 2）和IL-28B（IFN lambda 3），也被称为III型干扰素。异源二聚体受体，其一个亚基IL-10R2与超家族的其他成员共享。第二个亚基是IFNλR1或IL-28Rα，是IFNλ所特有的。尽管两个家族中细胞因子和受体之间的序列同源性很低，但通过Jak / STAT途径发生信号的方式与I型IFN（IFNα/β）类似，并激活了许多相同的基因。两种IFN家族均通过诱导靶细胞中抗病毒蛋白的产生以及MHC I类表达的上调而显示出抗病毒活性。这些蛋白质还表现出抗增殖和抗肿瘤作用，使其成为IFNα癌症疗法的可能替代品。与能够刺激大多数细胞的I型IFN不同，由于IFN lambda R1的表达有限，对IFN lambda刺激的反应似乎仅限于树突状细胞和某些肿瘤细胞。另一个显着差异是IFN lambda刺激驱动树突状细胞趋向于产生CD4 + CD25 + FoxP3 +调节性T细胞的能力，表明可能具有免疫调节作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。
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