IL-12 p70 Mouse Uncoated ELISA Kit/小鼠IL-12 p70未包被ELISA试剂盒
货号：88-7121-77,88-7121-88
规格：20X96 Tests,10X96 Tests
价格：8846,6261
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15 pg/mL |
| 检测范围 | 15-2,000 pg/mL |
样本类型/体积 | 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -ELISA/ELISPOT包被缓冲液粉末 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) 底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-44，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-12 p70 |
| Protein aliases | CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12A, IL-12B, Interleukin-12 subunit alpha, Interleukin-12 subunit beta |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Il-12a, Il-12b, IL-12p35, Il-12p40, Il12a, Il12b, Il12p40, Ll12a, p35, p40 |
| Gene ID | (Mouse) 16159,(Mouse) 16160 |
| Gene symbol | Il12a, Il12b |
| UniProt ID | (Mouse) P43431,(Mouse) P43432 |
The Mouse Interleukin 12 p70 (IL-12 p70) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-12 p70. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白细胞介素12 p70（IL-12 p70）未包被ELISA试剂盒包含预先匹配的抗体对，以及用于进行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠IL-12 p70的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.白介素12（IL-12）是一种异源二聚体70 kDa细胞因子，由两条共价连接的糖基化链组成，分子量分别为40kD（p40）和35-kD（p35）。 IL-12主要由单核细胞，巨噬细胞和树突状细胞响应细菌产品（如脂多糖（LPS）），细胞内病原体或与活化T细胞相互作用而产生。最初发现IL-12是因为它具有诱导干扰素-γ（IFN-g）产生，细胞增殖以及由自然杀伤细胞和T细胞介导的细胞毒性的能力。研究表明，IL-12在Th1反应的发展中也起着关键作用，导致IFN-g和IL-2的产生。这些细胞因子可进而促进T细胞反应和巨噬细胞活化。使用双启动子表达系统，在杆状病毒感染的昆虫细胞中产生重组小鼠IL-12 p70，作为前体p35和p40亚基的真正异源二聚体。 IL-12 p70与其他可用形式的蛋白质不同，因为它表达为真正的异源二聚体，而不是其中亚基通过人工接头连接的单链伪异源二聚体。淋巴细胞对此细胞因子的反应是由转录蛋白STAT4的激活剂介导的。发现一氧化氮合酶2A（NOS2A / NOS2）是先天免疫中该细胞因子信号传导过程所必需的。人和小鼠IL-12 p40共有约70％的氨基酸序列同源性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。
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