IL-27 Mouse Uncoated ELISA Kit/小鼠IL-27未包被ELISA试剂盒

货号：88-7274-88

规格：10 x 96 tests

价格：6261

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	16 pg/mL
检测范围	16-2,000 pg/mL
样本类型/体积	血清 100 μL 上清液 100 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -ELISA/ELISPOT包被缓冲液：10X PBS ELISA 包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) 底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-44，-76，-86）
储存温度	2-8℃
Protein name	IL-27 p28
Protein aliases	IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27-A, p28, interleukin 30, Interleukin-27 subunit alpha
物种（Tested）	小鼠
试剂盒类型	Uncoated ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-27, IL-27p28, Il27, Il27a, Il30, p28
Gene ID	(Mouse) 246779
Gene symbol	Il27
UniProt ID	(Mouse) Q8K3I6

The Mouse Interleukin 27 (IL-27) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-27. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素27（IL-27）未包被的ELISA试剂盒包含预先匹配的抗体对，以及用于进行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠IL-27的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-γ-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rβ2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.IL-27是IL-12家族的成员，是一种异二聚体蛋白，由p40相关蛋白爱泼斯坦-巴尔病毒诱导的基因3（EBI3）非共价连接到IL-12p35相关蛋白p28（也称为IL-30）。 IL-27由活化的APC和成熟的树突状细胞产生。 IL-27在NK细胞和天然CD4 + T细胞上发挥活性。 IL-27受体成分（WSX-1 / TCCR和gp130）的mRNA表达分析表明，IL-27也可能靶向其他细胞，包括肥大细胞和单核细胞。 IL-27与WSX-1 / gp130的结合可激活JAK1，STAT1和STAT3和STAT1 / 3磷酸化。 WSX-1 / TCCR缺陷型小鼠的Th1应答受损，并且更易感染单核细胞增生李斯特菌，提示Th1应答需要IL-27。尽管启动Th1响应需要激活WSX-1，但并不需要维持Th1响应。单独的IL-27不能诱导CD4 + T细胞分化为产生IFN-γ的细胞，提示IL-27作为Th1应答的初始激活物。 IL-27在引发Th1反应中的重要作用是诱导Th1特异性转录因子T-bet以及抑制Th2特异性转录因子GATA-3。 T-bet通过在CD4 + T细胞活化后维持IL-12Rβ2表达的能力在Th1分化中起关键作用。最近的研究表明IL-27具有有效的抗肿瘤活性。在体外，已经发现IL-27直接作用于幼稚的CD8细胞，产生具有增强的粒酶B表达的CTL。在体内，据报道IL-27增强CTL活性，抑制肿瘤生长，并诱导原发性和转移性神经母细胞瘤完全消退。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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