Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488/山羊抗小鼠IgG (H+L)超级克隆重组二抗，Alexa Fluor 488
货号：A28175
规格：1 mg
价格：3319
产品类型：荧光二抗
品牌：Thermo Fisher
抗原：Recombinant full-length protein
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：Alexa Fluor 488
点击查看所有Alexa Fluor 荧光二抗
| 抗体类型: | 荧光二抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 1-5 µg/mL (Flow)； 1 µg/mL (ICC)； 1 µg/mL (IF);1:2000(IHC(F)) |
产品详细信息
The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
数据 |
| Mouse IgG (H+L) Secondary Antibody (A28175) in IF Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L)/IgM (L) Secondary Antibody Alexa Fluor® 488 conjugate (Product # A28175) was used at a concentration of 1 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.Mouse IgG (H+L) Secondary Antibody (A28175) in IHC (F)Immunofluorescence analysis of Cardiac Troponin T in mouse heart tissue: Frozen sections were fixed with 4% paraformaldehyde for 20 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 10% Normal goat serum for 1 hour at room temperature. Whole heart longitudinal sections were then incubated with Cardiac Troponin T Mouse Monoclonal Antibody (Product# MA5-12960, 5µg/mL) overnight at 4°C, followed by Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175, 1:2000,45 mins). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and cytoskeletal F-actin (red) was stained using Rhodamine Phalloidin (Product # R415, 1:300). Panel a) represents staining with the matched isotype control. Panel b) shows representative sections stained for Cardiac Troponin T (green). The images were captured at 20X magnification.Mouse IgG (H+L) Secondary Antibody (A28175) in IF Immunofluorescence analysis of Neurofilament, Light chain was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurofilament, Light chain (DA2) Mouse Monoclonal Antibody (Product # MA1-2010) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. |
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参考文献： |
| 1. eLifeInhibitor of ppGalNAc-T3-mediated O-glycosylation blocks cancer cell invasiveness and lowers FGF23 levels.2. Cancer medicineDual pharmacological inhibition of glutathione and thioredoxin systems synergizes to kill colorectal carcinoma stem cells.3. OncotargetCombined genetic and epigenetic interferences with interferon signaling expose prostate cancer cells to viral infection.4. Nature neuroscienceA neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination.5.Experimental and therapeutic medicineTherapeutic effects of triptolide via the inhibition of IL-1β expression in a mouse model of ulcerative colitis. |
产品应用 Flow;ICC;IF;IHC(F);IHC(P);WB;IHC
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