IgG Subclass Human ELISA Kit/人IgG Subclass ELISA试剂盒

货号：991000

规格：2 x 96 tests

价格：0

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：IgG

荧光染料：其它

样本类型/体积	血清 10 μL
操作时间	20 min
测试时间	1 hr 10 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	2X 96 Tests
组份	- 预包被96孔板- 标准品- 样品稀释液- 检测缓冲浓缩液- 血清对照- IgG亚类的单克隆抗体（4）- 过氧化物酶偶联抗体- 洗涤液- 底物- 终止液- 胶板盖
储存温度	2-8℃
Protein name	Human IgG
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP

The Human IgG Subclass Profile (Hu IgG) ELISA quantitates Hu IgG subclasses in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu igG1, IgG2, IgG3 or IgG4.人IgG亚类（Hu IgG）ELISA定量了人血清或细胞培养基中的Hu IgG亚类。 该检测试剂盒将专门识别天然和重组Hu igG1，IgG2，IgG3或IgG4。Principle of the method 方法原理The Human IL-10 US solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-10 US固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，该第二抗体与与捕获抗体不同的表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody’s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.一抗的同种型及其应用可能导致背景染色。一抗背景噪音可能是由于与靶细胞上的Fc受体结合而引起的。通过与细胞蛋白质，碳水化合物和脂质的非特异性相互作用；或通过细胞自发荧光。同型对照抗体可以充当阴性对照，以帮助区分非特异性背景信号和特异性抗体信号，因为它们对靶抗原没有相关特异性。尽管同型对照最常用于流式细胞术，但它们可用于其他应用，例如染色质免疫沉淀（ChIP），免疫组织化学和凝胶迁移。同型对照应与一抗的种类和同种型匹配，以便可以准确确定一抗特异性染色的水平。如果使用直接标记的第一抗体，则同型对照如果与测试抗体标记相同，则效果最佳。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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