Rat anti human TNF alpha: PE
货号：MCA1560PE
规格：100 Tests
价格：3740
产品类型：流式抗体
品牌：Bio-Rad
抗原：TNF alpha
物种：人
宿主：大鼠
抗体亚型：IgG1
克隆号：MP9-20A4
荧光染料：PE
| 产品介绍 | | |
| Rat anti Human TNF alpha antibody, clone MP9-20A4recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This ~17kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells.Rat anti Human TNF alpha antibody, clone MP9-20A4 is reported to act as a blocking antibody (Abramset al.2001). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide 1%Bovine Serum Albumin 5% Sucrose |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4°C.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend micro-centrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. The use of Leucoperm (Product CodeBUF09) is recommended for this purpose. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG1 Negative Control:RPE | MCA6004PE | Flow | 100 tests |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | Flow | 50 tests |
| Human Seroblock | BUF070B | Flow | 200 tests |
| 文献 |
| 1. Butler, D.M.et al.(1997) DBA/1 mice expressing the human TNF-alpha transgene develop a severe, erosive arthritis: characterization of the cytokine cascade and cellular composition.J Immunol. 159 (6): 2867-76.2. Vujanovic, L.et al.(2010) Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15.Blood. 116: 575-83.3. Abrams, J.S. (2001) Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.Curr Protoc Immunol. Chapter 6: Unit 6.20.4. Evans HGet al.(2009)In vivoactivated monocytes from the site of inflammation in humans specifically promote Th17 responses.Proc Natl Acad Sci U S A. 106 (15): 6232-7.5. Kobawala TPet al.(2016) Significance of TNF-α and the Adhesion Molecules: L-Selectin and VCAM-1 in Papillary Thyroid Carcinoma.J Thyroid Res. 2016: 8143695.6. Mann, E.R.et al.(2016) Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum.Gut. 65 (2): 256-70.7. Kumagai, M.et al.(2012) Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.J Control Release. 160 (3): 542-51.8. Poussin Cet al.(2014) Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in anin vitroassay revealed by transcriptomics analysis.Toxicol In Vitro. 28 (5): 896-908. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
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