F(ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, Qdot 565/F(ab')2-山羊抗大鼠 IgG (H+L) 二抗, Qdot 565

货号：Q-11631MP

规格：200 µL

价格：6881

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Qdot 565

类型:	二抗	同型对照：	IgG
浓度：		用法：	1:50(Flow);1:1000(ICC);1:1000(IF);1:50(WB);1:50(IHC)

产品详细信息Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG (H+L) Secondary Antibody (Q-11631MP) in IFImmunofluorescence analysis of F (ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, Qdot® 565 was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/mL Rat primary antibody for 3 hours at room temperature. F (ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, Qdot® 565 (Product # Q-11631MP) was used at 1:1000 dilution in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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