Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550/山羊抗大鼠 IgG (H+L) 交叉吸附 二抗, DyLight 550

货号：SA5-10019

规格：500 µg

价格：2311

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：山羊

抗体亚型：IgG

荧光染料：DyLight 550

类型:	二抗	同型对照：	IgG
浓度：	0.5 mg/mL	用法：	1:50-1:200（Flow）;2.0 µg/mL(ICC);10 µg/mL(IF);1:50-1:2,000(IHC);1:5,000-1:20,000(WB)

产品详细信息This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, human, mouse, rabbit and sheep.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10019) in IFImmunofluorescence analysis of Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/mL Rat primary antibody for 3 hours at room temperature. Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 (Product # SA5-10019) was used at a concentration of 2µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21171.htm