Chicken anti-Rat IgG (H+L) Secondary Antibody, Biotin/鸡抗大鼠 IgG (H+L) 二抗, Biotin

货号：PA1-30361

规格：1 mg

价格：6026

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：其它

荧光染料：Biotin

类型:	二抗	同型对照：	IgG
浓度：	1.0 mg/mL	用法：

产品详细信息PA1-30361 detects Rat IgG from rat samples.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG (H+L) Secondary Antibody (PA1-30361) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and NCCIT (Lane 2). The blots were probed with Anti-Alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected by chemiluminescence using Chicken anti-Rat IgG Secondary Antibody, Biotin (Product # PA1-30361) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:50,000 (Fig. 3). A 52 kDa band corresponding to Alpha Tubulin was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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