Mouse anti-Rat IgG2a Secondary Antibody, FITC/小鼠抗大鼠 IgG2a 二抗, FITC

货号： SA1-25265

规格：125 µg

价格：4057

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：小鼠

抗体亚型：IgG

荧光染料：FITC

类型:	二抗	同型对照：	IgG2a
浓度：	1.0 mg/mL	用法：	5 µg/mL for 10^6 cells in 100 µL(Flow)

产品详细信息SA1-25265 detects rat IgG2a, no cross reactivity is observed with other rat immunoglobulin subclasses.Use 10 µL of the suggested dilution to label 10^6 cells in 100 µL.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG2a Secondary Antibody (SA1-25265) in FlowFlow cytometry analysis of CCR7 on primary mouse splenocytes. Cells were blocked with an anti-CD16/32 monoclonal antibody (Fc receptor blocker) for 30 minutes on ice, and then stained with a CCR7 monoclonal antibody (Product # MA1-163), using 5 µg of antibody per 1x106 cells, in 1X PBS containing 5% FCS. After incubation of the primary antibody for 1 hour at 37C, the cells were stained with a FITC-conjugated mouse anti-rat IgG2a secondary antibody (Product # SA1-25265) for at least 30 minutes on ice. A representative 20,000 cells were acquired for each sample, and the appropriate FSC vs SSC gate (left panel) was used to assess CCR7 staining on lymphocytes (right panel). The cells were also stained with a PE-conjugated hamster anti-mouse CD3 epsilon monoclonal antibody.

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