Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800/驴抗大鼠 IgG (H+L) 交叉吸附二抗, DyLight 800

货号：SA5-10032

规格：500 µg

价格：2335

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：驴

抗体亚型：IgG

荧光染料：DyLight 800

类型:	二抗	抗原：	Rat IgG-heavy and light chain
浓度：	0.5 mg/mL	用法：	1:25 - 1:100(Flow);1:50-1:2,000(ICC);1:50-1:2,000(IF);1:50-1:2,000(IHC);1:5,000-1:20,000(WB)

产品详细信息This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, goat, human, mouse, rabbit and sheep.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10032) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of L6 (Lane 1) and F9 (Lane 2). The blots were probed with Anti-alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected using Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800 (Product # SA5-10032) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 52 kDa band corresponding to alpha Tubulin was observed across the cell lines tested. Alpha Tubulin was multiplexed with Anti-GAPDH Mouse Monoclonal antibody (Product # MA5-15738-1MG, 1:4000 dilution) and detected using Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 680 (Product # A-21057, 0.2 µg/mL). A 37 kDa band corresponding to GAPDH was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibodies after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).

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