| alpha Tubulin Antibody (13-8000) in IFImmunofluorescent analysis of Alpha-Tubulin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Alpha-Tubulin Mouse monoclonal Antibody (Product # 13-8000) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 20X magnification.alpha Tubulin Antibody (13-8000) in IHC (P)Immunohistochemistry analysis of Alpha-Tubulin showing staining in the cytoplasm of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Alpha-Tubulin monoclonal antibody (Product # 13-8000) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. |
| 1.EMBO reportsRab7b modulates autophagic flux by interacting with Atg4B."13-8000 was used in Immunofluorescence to show that the cysteine protease Atg4B, a key enzyme in autophagy that cleaves LC3, is an interactor of the small GTPase Rab7b."AuthorsKjos I,Borg Distefano M,Sætre F,Repnik U,Holland P,Jones AT,Engedal N,Simonsen A,Bakke O,Progida C2.Liver international : official journal of the International Association for the Study of the LiverA possible involvement of p62/sequestosome-1 in the process of biliary epithelial autophagy and senescence in primary biliary cirrhosis."13-8000 was used in western blot to investigate autophagy and senescence in primary biliary cirrhosis."AuthorsSasaki M,Miyakoshi M,Sato Y,Nakanuma Y3.Neurobiology of diseaseHeterogeneous intrastriatal pattern of proteins regulating axon growth in normal adult human brain."13-8000 was used in western blot to assess the distribution of axon growth markers in mature human striatum"AuthorsTong J,Furukawa Y,Sherwin A,Hornykiewicz O,Kish SJ4.Experimental cell researchIncreased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells."13-8000 was used in immunocytochemistry, immunoprecipitation, and western blot to report that CSE1L regulates the association of alpha-tubulin with beta-tubulin and promotes migration of MCF-7 breast cancer cells"AuthorsTai CJ,Shen SC,Lee WR,Liao CF,Deng WP,Chiou HY,Hsieh CI,Tung JN,Chen CS,Chiou JF,Li LT,Lin CY,Hsu CH,Jiang MC5.Molecular and cellular biochemistryFunction of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon."13-8000 was used in western blot to study the regulatory role of CSE1L/CAS in the secretion of human colorectal cells"AuthorsTsao TY,Tsai CS,Tung JN,Chen SL,Yue CH,Liao CF,Wang CC,Jiang MC |
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