IL-29 (IFN lambda 1) Human Uncoated ELISA Kit/人IL-29 (IFN lambda 1) 未包被ELISA试剂盒

货号：88-7296-77,88-7296-88

规格：20X96 Tests,10X96 Tests

价格：8846,6261

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	8 pg/mL
检测范围	8-1,000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -ELISA/ELISPOT包被缓冲液粉末 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB)溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-44，-76，-86）
储存温度	2-8℃
Protein name	IL-29
Protein aliases	Cytokine Zcyto21, IFN-lambda-1, Interleukin-29, IL-29, Interferon lambda-1, interferon, lambda 1, interferon-lambda-1, interleukin 29 (interferon, lambda 1)
物种（Tested）	人
试剂盒类型	Uncoated ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IFNL1, IL-29, IL29, ZCYTO21
Gene ID	(Human) 282618
Gene symbol	IFNL1
UniProt ID	(Human) Q8IU54

The Human Interleukin 29 (IFN lambda 1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-29. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素29（IFNλ1）未包被ELISA试剂盒包含预先匹配的抗体对，以及用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量人IL-29的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity.干扰素-λ构成了干扰素的新家族，其介导了多种细胞中抗病毒保护的诱导。该家族中有3个成员，分别是lambda1，lambda2和lambda3，分别称为IL-29，IL-28A和IL-28B。类似于I型IFN，IFN-λ激活细胞内信号传导以促进基因表达。 IFN-λ通过II类细胞因子受体复合物介导其抗病毒保护作用，该复合物由两种必需的受体蛋白CRF2-12 /IFN-λR1（对于IFN-λ而言是独特的）和CFR2-4 / IL-10R2组成。与IL-10，IL-22和IL-26受体共有。最近已经报道了IFN-λ，IL-28和IL-29引发树突状细胞，从而诱导带有Foxp3的调节性T细胞的增殖。响应IFN-λ分化的DC表达高水平的I和II类MHC基因产物，但表达低水平的共刺激分子。而且，这些细胞可以特异性地诱导具有接触依赖性抑制活性的CD4 + CD25 + FOXP3 + T细胞的IL-2依赖性增殖。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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