IL-12/IL-23 p40 (Total) Mouse Uncoated ELISA Kit/小鼠IL-12/IL-23 p40 (Total) 未包被ELISA试剂盒

货号：88-7120-77,88-7120-88

规格：20X96 Tests,10X96 Tests

价格：8846,6261

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2 pg/mL
检测范围	2-300 pg/mL
样本类型/体积	血清 100 μL 上清液 100 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -ELISA/ELISPOT包被缓冲液粉末 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB)溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-44，-76，-86）
储存温度	2-8℃
Protein name	IL-12/IL-23 p40
Protein aliases	CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, IL-23 subunit alpha, IL-23-A, IL-23p19, Interleukin-12 subunit beta, Interleukin-23 subunit alpha, Interleukin-23 subunit p19
物种（Tested）	小鼠
试剂盒类型	Uncoated ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	Il-12b, Il-12p40, IL-23, Il12b, Il12p40, Il23a, p19, p40
Gene ID	(Mouse) 16160,(Mouse) 83430
Gene symbol	Il12b, Il23a
UniProt ID	(Mouse) P43432,(Mouse) Q9EQ14

The Mouse IL-12/IL-23 p40 (Total) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-12/IL-23 p40 (Total). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠IL-12 / IL-23 p40（总）未包被ELISA试剂盒包含预匹配的抗体对，以及用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠IL-12 / IL-23的蛋白质水平 p40（总计）。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.IL-12是由于抗原刺激而由树突状细胞，嗜中性粒细胞，巨噬细胞和人淋巴母细胞产生的白介素。 IL-12是由IL-12A（p35）和IL-12B（p40）编码的异二聚体细胞因子。 IL-23是一种异二聚体细胞因子，与IL-12（IL-12B）亚基以及IL-12A（IL-12p19）亚基共享一个亚基。 在功能上，IL-12增加了血管内皮粘附分子-1在内皮上的表达，将白细胞引向肿瘤，并促进了肿瘤组织的缺血性出血性坏死。 相比之下，IL-23在刺激记忆性T细胞中起重要作用，并且是Th17细胞的诱导，扩增，维持和下游效应子功能所必需的，Th17细胞在上调中性粒细胞趋化因子和各种促炎性细胞因子中起着至关重要的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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