Granzyme B Mouse Uncoated ELISA Kit with Plates/小鼠Granzyme B ELISA试剂盒含未包被板

货号：88-8022-22,88-8022-86

规格：2X96 Tests,10X96 Tests

价格：3125,6537

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	40 pg/mL
检测范围	40-5,000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated,biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86）
储存温度	2-8℃
Protein name	Granzyme B
Protein aliases	CCP1, CTLA-1, Cytotoxic cell protease 1, Fragmentin-2, Granzyme B(G, H)
物种（Tested）	小鼠
试剂盒类型	Uncoated ELISA Kitwith Plates
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	AI553453, CCP-1/C11, CCP1, Ctla-1, Ctla1, GZB, Gzmb
Gene ID	(Mouse) 14939
Gene symbol	Gzmb
UniProt ID	(Mouse) P04187

The Mouse Granzyme B Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Granzyme B. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠Granzyme B未包被的ELISA试剂盒包含预先匹配的抗体对，平板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠Granzyme B的蛋白质水平。需要使用液和终止液来完成ELISA反应 并单独出售。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.GranzymeB是颗粒酶丝氨酸蛋白酶家族的成员，存在于细胞毒性T细胞和NK细胞的颗粒中。颗粒酶B已被描述为CGL1（组织蛋白酶G样-1），一种仅在细胞活化后在细胞毒性T淋巴细胞中表达的丝氨酸蛋白酶，而CTLA-1（与细胞毒性T淋巴细胞相关的丝氨酸酯酶1）则基于mRNA的鉴定。各种细胞毒性T细胞，但未在非细胞毒性淋巴样细胞中观察到。颗粒酶B对于通过凋亡快速诱导靶细胞死亡至关重要，凋亡是通过与细胞毒性T细胞相互作用诱导的。已经鉴定出参与该过程的受体是6-磷酸甘露糖受体，其在细胞毒性T细胞诱导的细胞凋亡期间充当粒酶B的死亡受体。颗粒酶B进入靶细胞以裂解caspase-3，并启动caspase级联反应，导致DNA片段化和凋亡。颗粒酶B还可以通过切割Bid蛋白来通过线粒体凋亡途径起作用。颗粒酶是中性的丝氨酸蛋白酶，它们存储在细胞毒性T淋巴细胞（CTL）的专门裂解颗粒和自然杀伤（NK）细胞中。已经从小鼠CTL和NK细胞中分离并克隆了许多颗粒酶（A至G），但是在人类中，已经克隆和鉴定的颗粒酶很少。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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