CD8a Monoclonal Antibody (53-6.7), FITC, eBioscience™
货号：11-0081-81 ,11-0081-82 ,11-0081-85,11-0081-86
规格：50 μg,100 μg,500 μg ,1 mg
价格：615,807,2432,4357
产品类型：流式抗体
品牌：eBioscience
抗原：CD8a
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：53-6.7
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| 浓度： | 0.5 mg/mL | 用法： | 0.5 µg/test (Flow） |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte or splenocyte suspensions. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
CD8a Antibody (11-0081-81) in FlowStaining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (open histogram) or 0.25 µg of Anti-Mouse CD8a FITC (filled histogram). Total viable cells were used for analysis.CD8a Antibody (11-0081-81) Cell regeneration (London, England) 2018 -Fig. 2 Flow cytometry analysis of the purity of sorted Ter119 - Mac1 - CD3 - CD4 - CD8 - B220 + CD19 + CD93 + IgM - pro/pre-B cells . Bone marrow nucleated cells were first incubated with biotin-conjugated anti-B220 antibody and then enriched by streptavidin microbeads using AutoMACS Pro. The pro/pre-B cells were further sorted from the B220 enriched cells by defined markers. Flow cytometry analysis confirmed the purities of B220 enriched cells (top) and sorted pro/pre-B cells (bottom).CD8a Antibody (11-0081-81) eLife 2018 -Figure 3--figure supplement 4. Thymocytes from homozygous mutant enhancer Bcl11b YFPDeltaEnh/YFPDeltaEnh mice are able to generate T-cell subsets expressing Bcl11b at normal levels relative to wild-type enhancer Bcl11b YFP/YFP mice. Representative FACS plots showing gates used for CD4 and CD8 double negative (DN), double positive (DP) and single positive (CD4 and CD8) populations (left plots) and the relative levels of Bcl11b-YFP in each subset generated from enhancer mutant and wild-type mice (right histograms, n = 2 for each genotype). |
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| 参考文献： |
| 1.Frontiers in immunology A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2+ T Cells.2.Oncoimmunology Identification of inhibitors of myeloid-derived suppressor cells activity through phenotypic chemical screening.3.Frontiers in neurology IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.4.Frontiers in physiology Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung.5.mSphere Microbiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice. |
技术参数 产品应用 Flow;IF;IHC;IHC(F);IP;ICC;W;Misc;FN
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