Mouse anti-Human IgM Secondary Antibody/鼠抗人IgM二抗

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Mouse anti-Human IgM Secondary Antibody/鼠抗人IgM二抗

货号:SA1-72512

规格:100 µg

价格:4039

产品类型:二抗

品牌:Thermo Fisher

抗原:Recombinant human IgM

物种:人

宿主:小鼠

抗体亚型:IgM

克隆号:KT16

荧光染料:其它

抗体类型: 二抗同型对照:

浓度:

1 mg/mL

用法:

5 µg/mL (ELISA)

产品详细信息This antibody reacts with the IgM chain C region and does not cross-react with human IgG and recombinant human IgG1, IgG2, IgG3, IgG4, IgA or IgE. This can be used in an Indirect ELISA (1 µg/mL) or as a capture antibody in a sandwich ELISA (5 µg/mL).靶标信息Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
数据

Human IgM Secondary Antibody (SA1-72512) in ELISASandwich ELISA analysis of human IgM using a Mouse anti-Human IgM secondary antibody (Product # SA1-72512). 5 µg/ml of the Mouse anti-Human IgM antibody (Product # SA1-72512) was coated onto a plate as a capture antibody. Recombinant human IgM and recombinant human IgE (negative control) were added at the indicated concentrations. A Horseradishperoxidase(HRP) conjugated antibody at a dilution of 1:1000 was used as the detection antibody. The color was developed using substrate 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)

Human IgM Secondary Antibody (SA1-72512) in ELISASandwich ELISA analysis of Human IgM in Human serum using a Mouse anti-Human IgM secondary antibody (Product # SA1-72512). 5 µg/ml of the Mouse anti-Human IgM secondary antibody (Product # SA1-72512) was coated onto a plate as the capture antibody. Human serum was added at the indicated concentrations. A Horseradish peroxidase (HRP) conjugated antibody at a dilution of 1:1000 was used as the detection antibody. The color was developed using substrate 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)

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技术参数

产品应用 ELISA

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