F(ab')2-Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488/F(ab')2-兔抗小鼠 IgG (H+L)交叉吸附二抗，Alexa Fluor 488

货号：A-21204

规格：500 µg

价格：2787

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Alexa Fluor 488

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	0.2 µg/mL (ICC);0.2 µg/mL (IF)

靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据：

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21204) in IF Immunofluorescence analysis of F (ab')2-Rabbit anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 (Product # A-21204) was performed using MCF-7 cells stained with Cytokeratin 19 Mouse Monoclonal Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with mouse primary antibody (1:250 dilution) for 3 hours at room temperature. F (ab')2-Rabbit anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 was used at concentration of 0.2 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of cytokeratin 19 in the membrane (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1.Journal of neuroscience researchGPNMB ameliorates mutant TDP-43-induced motor neuron cell death.2.Molecular reproduction and developmentSimilar kinetics for 5-methylcytosine and 5-hydroxymethylcytosine during human preimplantation development in vitro.3.FASEB journal : official publication of the Federation of American Societies for Experimental BiologyVitamin D modulates tissue factor and protease-activated receptor 2 expression in vascular smooth muscle cells.4.The Journal of biological chemistryA region in urokinase plasminogen receptor domain III controlling a functional association with alpha5beta1 integrin and tumor growth.

技术参数

产品应用 ICC;IF;IHC(P);Misc

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