Rabbit anti-Human IgM Recombinant Secondary Antibody/兔抗人lgM重组二抗

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Rabbit anti-Human IgM Recombinant Secondary Antibody/兔抗人lgM重组二抗

货号:SA5-10207

规格:100 µg

价格:2732

产品类型:二抗

品牌:Thermo Fisher

抗原:Peptide corresponding to Human IgM.

物种:人

宿主:兔

抗体亚型:IgM

克隆号:RM121

荧光染料:其它

抗体类型: 二抗同型对照:
浓度: 1 mg/mL 用法:50-200 ng/well (Capture), 0.05-0.2 µg/mL (Detect)(ELISA) ;0.5-2 µg/mL(ICC) ;0.5-2 µg/mL (IF) ;0.5-2 µg/mL(IHC)
产品详细信息This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity andsensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

Human IgM Secondary Antibody (SA5-10207) in ELISAELISA of human immunoglobulins shows Rabbit Anti-Human IgM Secondary Antibody (Product # SA5-10207) reacts only to human IgM. No cross reactivity with IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of Rabbit Anti-Human IgM Secondary Antibody (Product # SA5-10207) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Human IgM Secondary Antibody (SA5-10207) in ELISAA titer ELISA using Rabbit Anti-Human IgM Secondary Antibody (Product # SA5-10207). The plate was coated with different amounts of human IgM. A serial dilution of Rabbit Anti-Human IgM Secondary Antibody (Product # SA5-10207) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody
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