Goat anti-Human IgG Fc Secondary Antibody, Biotin, eBioscience™

货号：13-4998-83

规格：200 µg

价格：1029

产品类型：二抗

品牌：Thermo Fisher

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

抗体类型:	二抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: This goat antibody reacts with the Fc fragment of heavy chains on human IgG but notwith the light chains on most human immunoglobulins. No antibody was detected against human IgM or IgA, or against nonimmunoglobulinserum proteins. This antibody has been tested to ensure minimalcross-reaction with bovine, horse, and mouse serum proteins, but the antibody may cross-react with immunoglobulins fromother species.Applications Reported: Biotin goat anti-Human IgG (Fcg Frag Specific) has been reported for use in flow cytometric analysis.Applications Tested: Biotin goat anti-Human IgG (Fc gamma Frag Specific) has been tested by flow cytometric analysis to detect purified human Fc-tagged proteins. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.We recommend a starting concentration of 0.03 µg of Streptavidin PE (cat. 12-4317) for detection of Biotin Anti-Human IgG Fcg Fragment Specific.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

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参考文献：

1.PloS oneDistinct Roles for CXCR6(+) and CXCR6(-) CD4(+) T Cells in the Pathogenesis of Chronic Colitis.2. Science translational medicineBroadly neutralizing human monoclonal JC polyomavirus VP1-specific antibodies as candidate therapeutics for progressive multifocal leukoencephalopathy.3. The Journal of clinical investigationInterferon-induced mechanosensing defects impede apoptotic cell clearance in lupus.

技术参数

产品应用 Flow;ELISA;IHC(F)

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