Epiandrosterone ELISA Kit/表雄酮ELISA试剂盒
货号：EIAANDO,EIAANDOX10
规格：96 tests,10X96 tests
价格：6143,54564
产品类型：ELISA检测
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.126 ng/mL |
| 检测范围 | 0.098-100 ng/mL |
样本类型/体积 | 血清 50 µL 血浆 50 µL 粪便提取物 50 µL 尿液 12.5 µL 上清液 50 µL |
操作时间 | 45 min |
测试时间 | 2 hr 30 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 16% |
| 批内变异系数（CV） | 8% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 抗-lgG包被96孔板 - 标准品 - 测定稀释浓缩液 - 捕获抗体 - HRP结合抗原 - 洗涤浓缩液 - TMB底物 - 终止液 - 胶板盖 |
储存温度 | -20°C |
| Protein name | Epiandrosterone |
物种（Tested） | 人 |
试剂盒类型 | Competitive ELISA |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| The Epiandrosterone Competitive ELISA quantitates epiandrosterone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Epiandrosterone Competitive ELISA research-use-only kit is designed to quantitatively measure epiandrosterone independent of species. An epiandrosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit epiandrosterone antibodies and epiandrosterone-peroxidase conjugate is added to the wells. The epiandrosterone-peroxidase conjugate and any epiandrosterone in the sample will compete to bind to the anti-rabbit epiandrosterone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound epiandrosterone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of epiandrosterone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Epiandrosterone竞争性ELISA定量分析血清，血浆，尿液，唾液，干燥的粪便提取物或细胞培养基中的表雄甾酮。|方法原理：Epiandrosterone竞争性ELISA研究专用试剂盒旨在定量测量与物种无关的表雄甾酮。提供了表雄甾酮标准品以生成测定的标准曲线，并从用户生成的标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗兔IgG抗体的透明微量滴定板中。将抗兔表雄甾酮抗体和表雄甾酮-过氧化物酶偶联物添加到孔中。表雄甾酮-过氧化物酶结合物和样品中的任何表雄甾酮将竞争结合抗兔表雄甾酮抗体。温育后，洗涤板并加入底物。底物与结合的表雄甾酮-过氧化物酶缀合物反应。孵育30分钟后，停止反应，并在微量滴定板读数器中于450 nm处检测所产生颜色的强度。产生的颜色强度与样品中表雄甾酮的含量成反比。|严格的验证：对该ELISA试剂盒的每个制造批次都进行了质量检测，测试标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Epiandrosterone is a dehydroepiandrosterone metabolite and a precursor of testosterone and estradiol with hypolipidemic and anabolic property. Epiandrosterone, a potential neurosteroid, appears to bind to the gamma-aminobutyric acid (GABA)/benzodiazepine-receptor complex (GABA-RC), acting as a negative non-competitive modulator of GABA-RC as well as signal through the N-methyl-D-aspartate receptor. In addition this agent inhibits the pentose phosphate pathway (PPP) thereby dilating blood vessels pre-contracted by partial depolarization. Also, epiandrosterone inhibits the synthesis of thromboxane A2 in activated platelets, reduces plasma plasminogen activator inhibitor type 1 and tissue plasminogen activator antigen, increases serum levels of insulin-like growth factor 1 and increases cyclic guanosine monophosphate and nitric oxide synthesis. These effects may improve circulation in the microvasculature. [National Center for Biotechnology Information. PubChem Compound Database; CID=441302].表雄酮是脱氢表雄酮的代谢产物，是具有降血脂和合成代谢特性的睾丸激素和雌二醇的前体。 Epiandrosterone，一种潜在的神经固醇，似乎与γ-氨基丁酸（GABA）/苯并二氮杂-受体复合物（GABA-RC）结合，可作为GABA-RC的负非竞争性调节剂，并通过N-甲基-D-天冬氨酸受体。另外，该试剂抑制了戊糖磷酸途径（PPP），从而扩张了由于部分去极化而预收缩的血管。此外，表雄甾酮抑制活化血小板中血栓烷A2的合成，降低血浆纤溶酶原激活物抑制剂1型和组织纤溶酶原激活物抗原，增加血清胰岛素样生长因子1的水平，并增加环鸟苷单磷酸和一氧化氮的合成。这些作用可以改善微脉管系统中的循环。 [国家生物技术信息中心。 PubChem化合物数据库； CID = 441302]。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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