Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody/羊抗人 IgG (H+L)交叉吸附二抗

货号：31119

规格：1.5 mg

价格：2274

产品类型：二抗

品牌：Thermo Fisher

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：其它

类型:	二抗	同型对照：
浓度：	1.8mg/mL	用法：	Assay Dependent (ICC)；Assay Dependent (ELISA)；Assay Dependent (IHC)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31119 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31119 reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse and mouse serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions. Country of origin: USA靶标信息Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Human IgG (H+L) Cross-Adsorbed Secondary Antibody (31119) in ELISASandwich ELISA analysis of human IgE was performed by coating wells of a 96-well plate with 100 µL per well of a goat anti-human IgG (H+L) antibody (Product # 31119) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382), and incubating overnight at 4C. The plate was washed and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) for at least 30 minutes at room temperature, and then 100 µL per well of human IgE, IgM, IgA, or IgG, diluted in carbonate/bicarbonate buffer (Product # 28382) at concentrations ranging from 1-1000 ng/mL, was added to the plate and incubated for at least 1 hour at room temperature. The plate was washed with 150 µL per well of 1X TBST, and 100 µL per well of a human IgE monoclonal antibody (Product # MA5-14704) was added to the wells at a concentration of 1 µg/mL, and incubated for 1 hour at room temperature. The plate was washed, and incubated with 100 µL per well of an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31439) at a dilution of 1:8000 for at least 30 minutes at room temperature. Detection was performed by adding 100 µL per well of 1-Step Ultra TMB substrate (Product # 34028) and incubating for a few minutes at room temperature. The reactions were then stopped with TMB Stop Solution (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.

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参考文献：

Nature communications Long-acting protein drugs for the treatment of ocular diseases.

技术参数

产品应用 ELISA；Flow；IF；ICC；IHC;IHC;WB

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