Autophagy Assay, Red Detection Kit
货号：APO010A,APO010B
规格：50 Tests,100 Tests
价格：3698,7820
产品类型：检测试剂盒
品牌：Bio-Rad
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| 产品介绍 | | |
| Autophagy Assay, Red Detection Kitallows for the detection and monitoring of in vitro development of autophagy in living cells.Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids and carbohydrates. Autophagy plays a critical role in maintaining homeostasis by preventing the accumulation of damaged organelles by disassembling unnecessary or dysfunctional cells and cellular components (Mizushima et al 2011). Autophagy occurs at low levels in the cell under normal conditions and can be rapidly upregulated during times of starvation or stress. Such degradation activities serve to provide nutrients (amino acids, nucleotides, fatty acids, etc.) and energy during periods of elevated bioenergetic demands (Mizushima et al 2011,Levine et al 2008). Another function of autophagy is to assist with the detection and destruction of intracellular pathogens (viruses, bacteria and parasites) (Levine et al 2011). Dysregulation of autophagy has been associated with many disease states including cancer, infection and degenerative diseases (Levine et al 2008). Autophagy is a dynamic process typically divided into three stages. During stage one, cytoplasmic components targeted for degradation are sequestered within a double-membrane phagopore (also called the isolation membrane). This results in the formation of a double-membrane vesicle called the autophagosome. During stage two, the autophagosome fuses with the lysosome to form the autolysosome. Degradation of the autophagosomal contents occurs during stage three (Mizushima et al 2011,Hundeshagen et al 2011). |
| 产品详情 | | |
| Test Principle | Autophagy Probe, Red is a cell-permeant aliphatic molecule that fluoresces brightly when inserted in the lipid membranes of autophagosomes and autolysosomes. Autophagy Probe, Red can be readily detected by flow cytometry with optimal excitation at 590 nm and peak emission at 620 nm (ZE5 Cell Analyzer settings, 561 nm laser and 615/24 or 640/20 filter). |
| Reagents in the Kit | Pack Size: 200 TestsAutophagy Probe, Red, 4 vials - lyophilizedFixative, 6 mlPack Size: 50 TestsAutophagy Probe, Red, 1 vial - lyophilizedFixative, 6 ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Red Probe | 590 | 620 |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL. Store the unopened kit (and each unopened component) according to the storage instructions on each component label.Store the Autophagy Probe, Red at -20℃. Once reconstituted in DMSO, the Autophagy Probe, Red stock should be stored at -20℃for 6 months, protected from light. Avoid repeated freezing and thawing. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Refer to Instructions For Use |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
技术参数 产品类型 flow
推荐用于 自噬检测
组成成分 flow
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