Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody/羊抗人IgG, IgM, IgA (H+L) 二抗

货号：31128

规格：2 mg

价格：2276

产品类型：二抗

品牌：Thermo Fisher

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：其它

类型:	二抗	同型对照：
浓度：	2.4 mg/mL	用法：	1 µg/mL(WB)；Assay Dependent(Flow)（ICC)(IHC)(IF)(IP)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31128 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31128 reacts with the heavy chains of human IgA, IgG, and IgM, and with the light chains common to most human immunoglobulins. The antibody does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions. Country of origin: USA靶标信息Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Human IgG, IgM, IgA (H+L) Secondary Antibody (31128) in WBWestern blot analysis of human IgE was performed by loading 0.5 µg of purified human IgE, IgM, IgA and IgG per well and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blots were blocked with 5% milk in TBST for at least 1 hour at room temperature. Human IgE was detected at ~75 kD using a human IgE monoclonal antibody (Product # MA5-14704) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse light chain secondary antibody at a dilution of 1:40,000 for at least 30 minutes at room temperature (left blot). To control for protein loading, a duplicate blot was probed with a mixture of goat anti-human IgG, IgM, and IgA (Product # 31128) and goat anti-human IgE (Product # A18801) antibodies at concentrations of 1 µg/mL, followed by a mouse anti-goat IgG-HRP secondary antibody (Product # 31400) at a dilution of 1:8000 (right blot). Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21555.htm